Plasmid_Backbone

Part:BBa_K2077001:Design

Designed by: Holly Cox   Group: iGEM16_RHIT   (2016-08-15)


pSB413-GPD: HIS3-selectable S. cerevisiae Expression Vector (RFC 10)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5807
    Illegal NheI site found at 1343
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 5813
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5807
    Illegal BglII site found at 1392
    Illegal BglII site found at 1452
    Illegal XhoI site found at 5148
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 5807
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 5807
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5822
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 603
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 3785
    Illegal SapI.rc site found at 2763
    Illegal SapI.rc site found at 4867


Design Notes

TBA


Source

TBA

References